Saturday, 15 June 2013

How to make a "Thin Section"

This post is about how thin section microscope slides are made, but before detailing the techniques used at Geosec it should be noted that there are many different techniques, and no one technique is correct, you just use the method that suits you. It is the finished item that is important, not how you get there.

I have several friends that make thin sections and we have an informal information exchange. We all use different methods, glues, machinery etc, and all produce good slides. I was never taught how to make slides, but found by trial and error a method that suits my requirements. My machines have been constructed in my workshop, and though crude by commercial standards they work well enough, and cost a fraction of the "standard" ones. All my slides are finished by hand, so any inaccuracy that comes from the machines is eliminated anyway.

So, Starting from a piece of rough rock, a small block 2cm x 2cm x 3cm is sawn by hand on a diamond saw.
The top surface need to be absolutely flat, so is lapped on a glass plate with fine silicon carbide grit.


Glue is applied and a microscope slide placed on the block. Any air bubbles in the glue are carefully worked out to the edge.


Geosec has moved from the traditional epoxy resin glues to UV setting ones, it is now necessary to put the slide/block under a hi-intensity UVlamp. The glue sets in a few seconds and is now ready to cut.


The slide/block is sawn as close to the glass as possible using a thin bladed diamond saw. The section is now 1mm to 0.5mm (500 microns) thick.


The slide is now mounted on to the first grinding machine. The slide is retained by vacuum in a slide holder and is held against a rotating diamond flat wheel. 


The arm reciprocates to ensure even cutting. This machine reduces the thickness to 200 microns.


The slide is transfered to the second grinding, or flat lap machine. 

 Using a finer grade diamond disc this can cut to almost the finished thickness, but there is always some score marks left by the cutting process, so the slides are removed when they are about 50 microns. The slide is now ready for hand finishing.  

The final stages are done by hand on a glass plate with silicon carbide grit, and repeated checking of the thickness by judging interference colours with the microscope.









2 comments:

  1. Dear
    What is the Glue that used ?????
    thanks

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  2. Hello Ahmed, I use loctite 350 for the sample to slide, and Loctite 358 for the coverslip. These are both UV setting, and may not suit all purposes, but they are good for reducing the number of bubbles. If you prefer to use epoxy I recommend ResinTech RT151. Hope this helps. Rob.

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